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验证数据展示

  • WB analysis using 83531-5-RR

    WB analysis using 83531-5-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83531-5-RR (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83531-5-RR

    FC experiment of HepG2 using 83531-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug METTL9 Recombinant antibody (83531-5-RR, Clone:240510A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83531-5-RR

    Affinity and Kinetic Characterization of 83531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83531-5-RR against Human METTL9 were performed. The affinity constant is below 1 pM.

METTL9 Recombinant antibody

METTL9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83531-5-RR

产品说明书

CloneNo. 240510A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

DREV1, DREV, CGI-81, CGI81, CGI 81

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83531-5-RR targets METTL9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 METTL9 fusion protein Ag7191 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 9
别名 DREV1, DREV, CGI-81, CGI81, CGI 81
计算分子量 37 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC000195
基因名称 METTL9
Gene ID (NCBI) 51108
RRIDAB_3671156
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H1A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

实验方案

Product Specific Protocols
WB protocol for METTL9 antibody 83531-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols