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验证数据展示

  • WB analysis of K-562 using 16791-1-AP (same clone as 16791-1-PBS)

    WB analysis of K-562 using 16791-1-AP (same clone as 16791-1-PBS)

    WB result of METTL5 antibody (16791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected K-562 cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 16791-1-AP (same clone as 16791-1-PBS)

    IP experiment of PC-3 using 16791-1-AP (same clone as 16791-1-PBS)

    IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • WB analysis using 16791-1-AP (same clone as 16791-1-PBS)

    WB analysis using 16791-1-AP (same clone as 16791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

    WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

    WB result of METTL5 antibody (16791-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

    WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

    WB result of METTL5 antibody (16791-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 16791-1-AP (same clone as 16791-1-PBS)

    IHC staining of mouse kidney using 16791-1-AP (same clone as 16791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL5 antibody included
  • IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

    IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL5 antibody included
  • IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

    IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL5 antibody included
  • IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

    IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

METTL5 Polyclonal antibody, PBS Only

METTL5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16791-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy METTL5 Ready-To-Use IHC Kit. METTL5 primary antibody included.

产品信息

16791-1-PBS targets METTL5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10232

Product name: Recombinant human METTL5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC000921

Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 5
别名 EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5
计算分子量 209 aa, 24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号BC000921
基因名称 METTL5
Gene ID (NCBI) 29081
RRIDAB_2142051
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRN9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.

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