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验证数据展示

  • WB analysis using 67733-2-Ig

    WB analysis using 67733-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67733-2-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67733-2-Ig

    WB analysis of HeLa using 67733-2-Ig

    WB result of METTL3 antibody (67733-2-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

  • IHC staining of human lung cancer using 67733-2-Ig

    IHC staining of human lung cancer using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included
  • Featured Product
  • KD/KO验证

METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67733-2-Ig

产品说明书

CloneNo. 4B4A3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67733-2-Ig targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag7110

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 229-580 aa of BC001650

Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 methyltransferase like 3
别名 IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
计算分子量 64 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
RRIDAB_2918503
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ86U44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
IHC protocol for METTL3 antibody 67733-2-IgDownload protocol
WB protocol for METTL3 antibody 67733-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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