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METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 15073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey and More (6)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (4)

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HT-1080 cells, mouse testis tissue, HepG2 cells, Jurkat cells, Neuro-2a cells, A549 cells, NCCIT cells, COS-7 cells, rat testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15073-1-AP targets METTL3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, RIP
经测试反应性 human, mouse, rat, monkey
文献引用反应性human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, drosophila
免疫原

CatNo: Ag7110

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 229-580 aa of BC001650

Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 3
别名 EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70
计算分子量 64 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
RRIDAB_2142033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86U44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 15073-1-APDownload protocol
IHC protocol for METTL3 antibody 15073-1-APDownload protocol
IF protocol for METTL3 antibody 15073-1-APDownload protocol
IP protocol for METTL3 antibody 15073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
mouseWB

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
  • KO Validated
humanWB

Nature

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

Authors - Junho Choe
  • KD Validated
humanWB

Nature

N(6)-methyladenosine-dependent regulation of messenger RNA stability.

Authors - Wang Xiao X
  • KD Validated
humanWB

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
  • KD Validated
mouseWB,IF

Science

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Authors - Shay Geula
  • KO Validated
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