验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, MCF-7 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
16983-1-AP targets METTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | METTL2 fusion protein Ag10410 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 2B |
| 别名 | methyltransferase like 2B, METL, METTL2, METTL2A, METTL2A/B, METTL2B, PSENIP1 |
| 计算分子量 | 313 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC107586 |
| 基因名称 | METTL2B |
| Gene ID (NCBI) | 55798 |
| RRID | AB_2923564 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P1Q9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for METTL2 antibody 16983-1-AP | Download protocol |
| IHC protocol for METTL2 antibody 16983-1-AP | Download protocol |
| IF protocol for METTL2 antibody 16983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response
| ||
J Inflamm Res Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis | ||
Brain Res Bull Rab3a attenuates spinal cord injury by mediating vesicle release
| ||
BMC Cancer METTL2B m3C RNA transferase: oncogenic role in ovarian cancer progression via regulation of the mTOR/AKT pathway and its link to the tumor immune microenvironment |
