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验证数据展示

  • WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB result of METTL14 antibody (80790-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected HeLa cells. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • Featured Product
  • KD/KO验证

METTL14 Recombinant monoclonal antibody, PBS Only

METTL14 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80790-1-PBS

产品说明书

CloneNo. 2E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy METTL14 Ready-To-Use IHC Kit. METTL14 primary antibody included.

产品信息

80790-1-PBS targets METTL14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14325

Product name: Recombinant human METTL14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 107-456 aa of BC007449

Sequence: LKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKCWTWDDIMKLEIDEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAVFQRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFCLGRRRLHLFGRDSTIRPGWLTVGPTLTNSNYNAETYASYFSAPNSYLTGCTEEIERLRPKSPPPKSKSDRGGGAPRGGGRGGTSAGRGRERNRSNFRGERGGFRGGRGGAHRGGFPPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 14
别名 2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit
计算分子量 456 aa, 52 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC007449
基因名称 METTL14
Gene ID (NCBI) 57721
RRIDAB_2918912
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCE5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.

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