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验证数据展示

  • WB analysis using 24379-1-AP (same clone as 24379-1-PBS)

    WB analysis using 24379-1-AP (same clone as 24379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24379-1-AP (METTL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24379-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 24379-1-AP (same clone as 24379-1-PBS)

    IF Staining of U-251 using 24379-1-AP (same clone as 24379-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using METTL13 antibody (24379-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24379-1-PBS in a different storage buffer formulation.

METTL13 Polyclonal antibody, PBS Only

METTL13 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24379-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

CGI 01, EEF1AKNMT, FEAT, KIAA0859, methyltransferase like 13

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24379-1-PBS targets METTL13 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19355

Product name: Recombinant human METTL13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 541-642 aa of BC029083

Sequence: QSDRMKVHIADGLDYIASLAGGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 13
别名 CGI 01, EEF1AKNMT, FEAT, KIAA0859, methyltransferase like 13
计算分子量 699 aa, 79 kDa
观测分子量72-78 kDa
GenBank蛋白编号BC029083
基因名称 METTL13
Gene ID (NCBI) 51603
RRIDAB_3742110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6R0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein methyltransferase like 13 (METTL13), also called FEAT, is coded by gene METTL13, which is located at chromosome 1q24.3. Purified from rat livers in 2011 by a Japanese researcher, METTL13 was found to be abnormally overexpressed in several human cancers including lung cancer and to drive tumorigenesis in transgenic mice. A study indicated that METTL13 inhibits apoptosis of lung, breast and liver cancer cells and miR-16 can repress the expression of METTL13 by binding to its 3′-untranslated region. (PMID: 33985542)

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