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验证数据展示

  • WB analysis using 33763-1-AP (same clone as 33763-1-PBS)

    WB analysis using 33763-1-AP (same clone as 33763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33763-1-AP (METTL10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33763-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 33763-1-AP (same clone as 33763-1-PBS)

    IHC staining of mouse brain using 33763-1-AP (same clone as 33763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33763-1-AP (METTL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33763-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 33763-1-AP (same clone as 33763-1-PBS)

    IF Staining of A431 using 33763-1-AP (same clone as 33763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using METTL10 antibody (33763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33763-1-PBS in a different storage buffer formulation.

METTL10 Polyclonal antibody, PBS Only

METTL10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33763-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C10orf138, EEF1AKMT2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33763-1-PBS targets METTL10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37000

Product name: Recombinant human METTL10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC026167

Sequence: MSSGADGGGGAAVAARSDKGSPGEDGFVPSALGTREHWDAVYERELQTFREYGDTGEIWFGEESMNRLIRWMQKHKIPLDASVLDIGTGNGVFLVELAKFGFSNITGIDYSPSAIQLSGSIIEKEGLSNIKLKVEDFLNLSTQLSGFHICIDKGTFDAISLNPDNAIEKRKQYVKSLSRVLKVKGFFSNNVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 10
别名 C10orf138, EEF1AKMT2
观测分子量26 kDa
GenBank蛋白编号BC026167
基因名称 METTL10
Gene ID (NCBI) 399818
RRIDAB_3743071
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5JPI9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methyltransferase-like 10 (METTL10) is a lysine trimethyltransferase targeting eukaryotic translation elongation factor 1A1 (eEF1A1), which is well preserved from simple eukaryotes such as Saccharomyces cerevisiae to mammals such as humans and mice. Recently, METTL10was identified as an estimated glomerular filtration rate (eGFR)-associated gene through a transethnic genome-wide association study of eGFR. (PMID: 39774961)

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