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验证数据展示

  • WB analysis using 16327-1-AP

    WB analysis using 16327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16327-1-AP (METRN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16327-1-AP

    IHC staining of mouse brain using 16327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16327-1-AP

    IHC staining of mouse brain using 16327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16327-1-AP

    IHC staining of mouse heart using 16327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16327-1-AP

    IHC staining of mouse heart using 16327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

METRN Polyclonal antibody

METRN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16327-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C16orf23, Meteorin, RJD6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16327-1-AP targets METRN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9414

Product name: Recombinant human METRN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-293 aa of BC000662

Sequence: YSEERCSWRGSGLTQEPGSVGQLALACAEGAVEWLYPAGALRLTLGGPDPRARPGIACLRPVRPFAGAQVFAERAGGALELLLAEGPGPAGGRCVRWGPRERRALFLQATPHQDISRRVAAFRFELREDGRPELPPQAHGLGVDGACRPCSDAELLLAACTSDFVIHGIIHGVTHDVELQESVITVVAARVLRQTPPLFQAGRSGDQGLTSIRTPLRCGVHPGPGTFLFMGWSRFGEARLGCAPRFQEFRRAYEAARAAHLHPCEVALH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 meteorin, glial cell differentiation regulator
别名 C16orf23, Meteorin, RJD6
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC000662
基因名称 METRN
Gene ID (NCBI) 79006
RRIDAB_3669238
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJH8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Meteorin, also named as METRN and C16orf23, is a secret protein which plays important roles in both glial cell differentiation and axonal network formation during neurogenesis.

实验方案

Product Specific Protocols
WB protocol for METRN antibody 16327-1-APDownload protocol
IHC protocol for METRN antibody 16327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols