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验证数据展示

  • WB analysis using 24640-1-AP

    WB analysis using 24640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24640-1-AP

    IHC staining of human colon cancer using 24640-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24640-1-AP (MEP1A antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MEP1A Polyclonal antibody

MEP1A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24640-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24640-1-AP targets MEP1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18950

Product name: Recombinant human MEP1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 365-713 aa of BC131714

Sequence: DDSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNSTGGIYLDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGYGFGLTLYPNSGESSGYLRLAFHVCSGENDAILEWPVENRQVIITILDQEPDVRNRMSSSMVFTTSKSHTSPAINDTVIWDRPSRVGTYHTDCNCFRSIDLGWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLSQTEVPTKGKRLSPQGLILQGQEQQVSEEGSGKAMLEEALPVSLSQGQPSRQKRSVENTGPLEDHNWPQYFRDPCDPNPCQNDGICVNVKGMASCRCISGHAFFYTGERCQAVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 meprin A, alpha (PABA peptide hydrolase)
别名 PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18
计算分子量 746 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC131714
基因名称 MEP1A
Gene ID (NCBI) 4224
RRIDAB_2879650
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16819
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MEP1A antibody 24640-1-APDownload protocol
IHC protocol for MEP1A antibody 24640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Comput Struct Biotechnol J

Integrated immunogenomic analysis of single-cell and bulk profiling reveals novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma

Authors - Saisai Tian
View Article