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验证数据展示

  • WB analysis using 14604-1-AP

    WB analysis using 14604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14604-1-AP (MEMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 14604-1-AP

    WB analysis of A549 using 14604-1-AP

    WB result of MEMO1 antibody (14604-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEMO1 transfected A549 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14604-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 14604-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14604-1-AP (MEMO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of BxPC-3 using 14604-1-AP

    IF Staining of BxPC-3 using 14604-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using MEMO1 antibody (14604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

MEMO1 Polyclonal antibody

MEMO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14604-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HCV NS5A-transactivated protein 7, CGI-27, CGI27, CGI 27, C2orf4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, A549 cells, rat testis tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14604-1-AP targets MEMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6151

Product name: Recombinant human MEMO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC070046

Sequence: MSNRVVCREASHAGSWYTASGPHLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQSSQCRNWQDSSVSYAAGALTVH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator of cell motility 1
别名 HCV NS5A-transactivated protein 7, CGI-27, CGI27, CGI 27, C2orf4
计算分子量 34 kDa
观测分子量 31-34 kDa
GenBank蛋白编号BC070046
基因名称 MEMO1
Gene ID (NCBI) 51072
RRIDAB_2297647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y316
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MEMO1 antibody 14604-1-APDownload protocol
IHC protocol for MEMO1 antibody 14604-1-APDownload protocol
WB protocol for MEMO1 antibody 14604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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