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MELK Recombinant antibody

MELK Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230106D4

Cat No : 82893-1-RR

Print datasheet

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

验证数据展示

  • FC experiment of MCF-7 using 82893-1-RR

    FC experiment of MCF-7 using 82893-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82893-1-RR targets MELK in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA
Tested Reactivity Human
Immunogen MELK fusion protein Ag1897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name maternal embryonic leucine zipper kinase
Synonyms hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38
Calculated Molecular Weight 651 aa, 75 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
FC protocol for MELK antibody 82893-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols