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MELK Polyclonal antibody

MELK Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11403-1-AP

Print datasheet

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

验证数据展示

  • WB analysis of Jurkat using 11403-1-AP

    WB analysis of Jurkat using 11403-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11403-1-AP

    IHC staining of human breast cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 11403-1-AP

    IHC staining of human breast cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11403-1-AP targets MELK in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MELK fusion protein Ag1897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name maternal embryonic leucine zipper kinase
Synonyms hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight 50-70 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
RRIDAB_2877762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
WB protocol for MELK antibody 11403-1-APDownload protocol
IHC protocol for MELK antibody 11403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

Int J Oncol

MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.

Authors - Jiecheng Ye
View Article
humanIHC

Med Sci Monit

Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.

Authors - Shaohan Wu
View Article