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验证数据展示

  • WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2K2 antibody included
  • IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP2K2 antibody included
  • IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK1/2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

MEK1/2 Monoclonal antibody, PBS Only

MEK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67410-1-PBS

产品说明书

CloneNo. 1H8C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

产品信息

67410-1-PBS targets MEK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29871

Product name: Recombinant human MAP2K2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC018645

Sequence: MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 2
别名 MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2
计算分子量 44 kDa
观测分子量 44-47 kDa
GenBank蛋白编号BC018645
基因名称 MEK2
Gene ID (NCBI) 5605
RRIDAB_2882651
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP36507
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.

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