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验证数据展示

  • IHC staining of human placenta using 31759-1-AP

    IHC staining of human placenta using 31759-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31759-1-AP (MEIKIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 31759-1-AP

    IF Staining of mouse testis using 31759-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using MEIKIN antibody (31759-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MDCK using 31759-1-AP

    IF Staining of MDCK using 31759-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using MEIKIN antibody (31759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MEIKIN Polyclonal antibody

MEIKIN Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 31759-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IF-P, ELISA

别名

LOC728637, similar to mCG13783

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inMDCK cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31759-1-AP targets MEIKIN in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36937

Product name: Recombinant human MEIKIN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of NM_001303622

Sequence: MWPLRVYTRKKREGQRLNLTPTPDLGSPAKAEAPPGSKRKGKVHGLSKIAEKAERSRQGGSGSGPFSPRLGVTGEKSLQENRSSEDTQDEKIASLRESVTDDLQVDSSSSNSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 similar to mCG13783
别名 LOC728637, similar to mCG13783
计算分子量373aa, 41 kDa
观测分子量40 kDa
GenBank蛋白编号NM_001303622
基因名称 LOC728637
Gene ID (NCBI) 728637
RRIDAB_3742474
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDA0A087WXM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Meikin is a conservative regulatory protein that plays a key role in meiosis. It was first discovered in mice. It is a meiotic-specific centromere protein and was named Meikin because of its important role in meiosis. Meikin's function is mainly realized by regulating the activity of Polo-like kinase PLK1. PLK1 is enriched to centromere under Meikin's dependence, which further affects the function of centromere.

实验方案

Product Specific Protocols
IF protocol for MEIKIN antibody 31759-1-APDownload protocol
IHC protocol for MEIKIN antibody 31759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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