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验证数据展示

  • WB analysis of mouse brain using 13456-1-AP

    WB analysis of mouse brain using 13456-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13456-1-AP (MEI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13456-1-AP

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of human cervical cancer using 13456-1-AP

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of rat testis using 13456-1-AP

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of rat testis using 13456-1-AP

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included

MEI1 Polyclonal antibody

MEI1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13456-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman cervical cancer tissue, human urothelial carcinoma tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MEI1 Ready-To-Use IHC Kit. MEI1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13456-1-AP targets MEI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4253

Product name: Recombinant human MEI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-238 aa of BC032248

Sequence: MLALTLAKADSPRTALLCSAWLLTASFSAQQHKGSLQVHQTLSVEMDQVLKALSFPKKKAALLSAAILCFLRTALRQSFSSALVALVPSGAQPLPATKDTVLAPLRMSQVRSLVIGLQNLLVQKDPLLSQACVGCLEALLDYLDARSPDIALHVASQPWNRFLLFTLLDAGENSFLRPEILRLMTLLQSMGHLADHSMAQTLQASLEGLPPSTSSGQPPLQDMLCLGGVAVSLSHIRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 meiosis inhibitor 1
别名 MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1
计算分子量 894 aa, 99 kDa
观测分子量 63 kDa
GenBank蛋白编号BC032248
基因名称 MEI1
Gene ID (NCBI) 150365
RRIDAB_2877951
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TIA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEI1 may play a role in meiosis during spermatogenesis. It is required for normal meiotic chromosome synapsis. MEI1 may be involved in the formation of meiotic double-strand breaks (DSBs) in spermatocytes. This antibody recognizes isoform 1, 2, 3, 5, and 7 of MEI1 gene with MW 141 kDa, 67-72 kDa,49-54 kDa and 26 kDa .

实验方案

Product Specific Protocols
WB protocol for MEI1 antibody 13456-1-APDownload protocol
IHC protocol for MEI1 antibody 13456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Res

Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.

Authors - Phillip J Hsu
View Article