验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HepG2 cells, U-251 cells, U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32300-1-AP targets MEGF8 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | MEGF8 fusion protein Ag36469 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | multiple EGF-like-domains 8 |
别名 | Multiple EGF-like domains protein 8, KIAA0817, Epidermal growth factor-like protein 4, EGF-like protein 4, EGFL4 |
计算分子量 | 303 kDa |
观测分子量 | 303 kDa |
GenBank蛋白编号 | NM_001271938.1 |
基因名称 | MEGF8 |
Gene ID (NCBI) | 1954 |
RRID | AB_3670233 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | Q7Z7M0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Multiple epidermal growth factor-like domains protein 8 (MEGF8, also known as C19orf49, EGFL4, and KIAA0817) is a multidomain transmembrane protein, which is known to interact with MGRN1, a protein that functions as an E3 ubiquitin ligase, and this interaction is involved in the regulation of Hedgehog signaling and heart development (PMID: 32966817). It is also involved in mediating Bone morphogenetic protein 4 (BMP4) signaling and guidance of developing trigeminal ganglion (TG) axons (PMID: 24052814). Immunoelectron microscopy showed that MEGF8 was present in the synapses and around the outer mitochondrial membrane, indicating its role in synaptic and mitochondrial functions (PMID: 38201267).
实验方案
Product Specific Protocols | |
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WB protocol for MEGF8 antibody 32300-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |