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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 32300-1-AP

    WB analysis using 32300-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32300-1-AP (MEGF8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MEGF8 Polyclonal antibody

MEGF8 Polyclonal Antibody for WB, ELISA
Cat No. 32300-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Multiple EGF-like domains protein 8, KIAA0817, Epidermal growth factor-like protein 4, EGF-like protein 4, EGFL4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, U-251 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32300-1-AP targets MEGF8 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MEGF8 fusion protein Ag36469 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 multiple EGF-like-domains 8
别名 Multiple EGF-like domains protein 8, KIAA0817, Epidermal growth factor-like protein 4, EGF-like protein 4, EGFL4
计算分子量303 kDa
观测分子量303 kDa
GenBank蛋白编号NM_001271938.1
基因名称 MEGF8
Gene ID (NCBI) 1954
RRIDAB_3670233
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z7M0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Multiple epidermal growth factor-like domains protein 8 (MEGF8, also known as C19orf49, EGFL4, and KIAA0817) is a multidomain transmembrane protein, which is known to interact with MGRN1, a protein that functions as an E3 ubiquitin ligase, and this interaction is involved in the regulation of Hedgehog signaling and heart development (PMID: 32966817). It is also involved in mediating Bone morphogenetic protein 4 (BMP4) signaling and guidance of developing trigeminal ganglion (TG) axons (PMID: 24052814). Immunoelectron microscopy showed that MEGF8 was present in the synapses and around the outer mitochondrial membrane, indicating its role in synaptic and mitochondrial functions (PMID: 38201267).

实验方案

Product Specific Protocols
WB protocol for MEGF8 antibody 32300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols