MEFV Polyclonal antibody
MEFV Polyclonal Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IF/ICC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, human plasma |
Positive IF detected in | A431 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24280-1-AP targets MEFV in WB, IP, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, WB, ELISA |
Cited Applications | WB, IP, IF, FC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | MEFV fusion protein Ag18000 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Mediterranean fever |
Synonyms | FMF, Marenostrin, Mediterranean fever, MEF, MEFV, Pyrin, TRIM20 |
Calculated Molecular Weight | 781 aa, 86 kDa |
Observed Molecular Weight | 86 kDa |
GenBank Accession Number | BC101511 |
Gene Symbol | MEFV |
Gene ID (NCBI) | 4210 |
RRID | AB_2879468 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O15553 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MEFV antibody 24280-1-AP | Download protocol |
IF protocol for MEFV antibody 24280-1-AP | Download protocol |
FC protocol for MEFV antibody 24280-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes | ||
Exp Ther Med Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock. | ||
PLoS Biol Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever |