验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells |
| Positive IP detected in | THP-1 cells |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24280-1-AP targets MEFV in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18000 Product name: Recombinant human MEFV protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC101511 Sequence: MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVQLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Mediterranean fever |
| 别名 | Pyrin |
| 计算分子量 | 781 aa, 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC101511 |
| 基因名称 | MEFV |
| Gene ID (NCBI) | 4210 |
| RRID | AB_2879468 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15553 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for MEFV antibody 24280-1-AP | Download protocol |
| IF protocol for MEFV antibody 24280-1-AP | Download protocol |
| IP protocol for MEFV antibody 24280-1-AP | Download protocol |
| WB protocol for MEFV antibody 24280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes | ||
Sci Immunol An N-terminal CDC42 T43I variant reveals the mechanism of pyrin inflammasome activation. | ||
Sci Immunol A genotype-first approach reveals the molecular basis of pyrin inflammasome activation. | ||
Biomaterials Dual-targeting nuclear and mitochondrial DNA damage drives immunogenic activation via PANoptosis for synergistic magneto-thermodynamic-chemotherapy. | ||
Acta Pharmacol Sin PYRIN m(6)A modification drives cardiomyocyte PANoptosis in sepsis-induced cardiomyopathy. |
