验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27452-1-AP targets MED9 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MED9 fusion protein Ag26158 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 9 |
| 别名 | MED25, MED9, mediator complex subunit 9 |
| 计算分子量 | 146 aa, 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC000647 |
| 基因名称 | MED9 |
| Gene ID (NCBI) | 55090 |
| RRID | AB_2880873 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWA0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED9, also named as MED25, is a 146 amino acid protein, which belongs to the Mediator complex subunit 9 family. MED9 is a component of the Mediator complex and a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED9 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED9 antibody 27452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |