验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Calu-3 cells, Daudi cells, MG-63 cells, PC-3 cells, T-47D cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33458-1-AP targets MED7 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38214 Product name: Recombinant human MED7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC006968 Sequence: MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEKDAALCVLIDEMNERP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 7 |
| 别名 | Mediator complex subunit 7, Activator-recruited cofactor 34 kDa component, ARC34, Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9 |
| 计算分子量 | 233 aa, 27 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC006968 |
| 基因名称 | MED7 |
| Gene ID (NCBI) | 9443 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43513 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED7 is a crucial subunit of the Mediator complex, a protein complex that regulates gene transcription by interacting with RNA polymerase II.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED7 antibody 33458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
