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验证数据展示

  • WB analysis using 15338-1-AP (same clone as 15338-1-PBS)

    WB analysis using 15338-1-AP (same clone as 15338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 15338-1-AP (same clone as 15338-1-PBS)

    IHC staining of human prostate cancer using 15338-1-AP (same clone as 15338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

MED6 Polyclonal antibody, PBS Only

MED6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15338-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15338-1-PBS targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7541

Product name: Recombinant human MED6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-246 aa of BC004106

Sequence: MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 6
别名
计算分子量 28 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC004106
基因名称 MED6
Gene ID (NCBI) 10001
RRIDAB_2142554
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75586
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

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