验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17652-1-AP targets MED27 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MED27 fusion protein Ag11897 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 27 |
| 别名 | Cofactor required for Sp1 transcriptional activation subunit 8, CRAP34, CRSP34, CRSP8, MED 27 |
| 计算分子量 | 311 aa, 35 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC064608 |
| 基因名称 | MED27 |
| Gene ID (NCBI) | 9442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P2C8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED27 is a subunit of the Mediator complex, a large multiprotein complex that acts as a bridge between DNA-binding transcription factors and the RNA polymerase II transcriptional machinery. It is involved in the regulation of gene transcription and is essential for the initiation of transcription. The protein is highly conserved across different species, highlighting its fundamental role in cellular processes. It also influences cellular growth, development, and response to environmental cues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED27 antibody 17652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |