MED23 Polyclonal antibody
MED23 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27850-1-AP targets MED23 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Immunogen | MED23 fusion protein Ag27241 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mediator complex subunit 23 |
| Synonyms | ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2 |
| Calculated Molecular Weight | 156 kDa |
| Observed Molecular Weight | 140 kDa, 50 kDa |
| GenBank Accession Number | BC060759 |
| Gene Symbol | MED23 |
| Gene ID (NCBI) | 9439 |
| RRID | AB_2880994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED23 antibody 27850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. |