验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, A375 cells, U2OS cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
27850-1-AP targets MED23 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | mouse |
免疫原 | MED23 fusion protein Ag27241 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | mediator complex subunit 23 |
别名 | MED23/CRSP3, CRSP133, CRSP130, CRSP complex subunit 3, Cofactor required for Sp1 transcriptional activation subunit 3 |
计算分子量 | 156 kDa |
观测分子量 | 140 kDa, 50 kDa |
GenBank蛋白编号 | BC060759 |
基因名称 | MED23 |
Gene ID (NCBI) | 9439 |
RRID | AB_2880994 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9ULK4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
实验方案
Product Specific Protocols | |
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WB protocol for MED23 antibody 27850-1-AP | Download protocol |
IHC protocol for MED23 antibody 27850-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |