验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
17598-1-AP targets MED20 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11598 Product name: Recombinant human MED20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-212 aa of BC040950 Sequence: MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 20 |
| 别名 | hTRFP, MED 20, Mediator of RNA polymerase II transcription subunit 20, TRF-proximal protein homolog |
| 计算分子量 | 212 aa, 23 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC040950 |
| 基因名称 | MED20 |
| Gene ID (NCBI) | 9477 |
| RRID | AB_2142162 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H944 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED20(Mediator of RNA polymerase II transcription subunit 20) is also named as TRFP and belongs to the Mediator complex subunit 20 family. The deduced 209-amino acid protein shares 44% amino identity with Drosophila Trfp. TRFP had an apparent molecular mass of 28 kD by SDS-PAGE. It is identified TRFP as a component of an RNA polymerase II-SRB complex. The TRFP-containing complex could substitute for core RNA polymerase II in driving basal transcription from a minimal promoter.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED20 antibody 17598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Surplus fatty acid synthesis increases oxidative stress in adipocytes and lnduces lipodystrophy | ||
J Virol Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. | ||
Cell Rep The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.
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