验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20029-1-AP targets MED13-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 13 |
| 别名 | ARC250, DRIP250, HSPC221, KIAA0593, MED13, MED13-Specific, mediator complex subunit 13, THRAP1, TRAP240 |
| 计算分子量 | 239 kDa |
| 观测分子量 | 240-250 kDa |
| GenBank蛋白编号 | NM_005121 |
| 基因名称 | MED13 |
| Gene ID (NCBI) | 9969 |
| RRID | AB_3085595 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHV7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED13, also named as ARC250, KIAA0593, THRAP1 and TRAP240, belongs to the Mediator complex subunit 13 family. MED13 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody is specific to MED13. It has no cross reaction to MED13L.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED13-Specific antibody 20029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |