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验证数据展示

  • WB analysis using 31041-1-AP

    WB analysis using 31041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31041-1-AP (MED12L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 31041-1-AP

    IF Staining of U2OS using 31041-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MED12L antibody (31041-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MED12L Polyclonal antibody

MED12L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31041-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA1635, Mediator complex subunit 12-like protein, Mediator of RNA polymerase II transcription subunit 12-like protein, PRO0314, Thyroid hormone receptor-associated-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31041-1-AP targets MED12L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MED12L fusion protein Ag34636 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 12-like
别名 KIAA1635, Mediator complex subunit 12-like protein, Mediator of RNA polymerase II transcription subunit 12-like protein, PRO0314, Thyroid hormone receptor-associated-like protein
计算分子量240 kDa
观测分子量260 kDa
GenBank蛋白编号NM_053002
基因名称 MED12L
Gene ID (NCBI) 116931
RRIDAB_3669826
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED12L antibody 31041-1-APDownload protocol
IF protocol for MED12L antibody 31041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols