验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20047-1-AP targets MED12L in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 12-like |
| 别名 | KIAA1635, Mediator complex subunit 12-like protein, Mediator of RNA polymerase II transcription subunit 12-like protein, PRO0314, Thyroid hormone receptor-associated-like protein |
| 计算分子量 | 240 kDa |
| 观测分子量 | 260-280 kDa |
| GenBank蛋白编号 | NM_053002 |
| 基因名称 | MED12L |
| Gene ID (NCBI) | 116931 |
| RRID | AB_10734594 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86YW9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED12L antibody 20047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
