验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20028-1-AP targets MED12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 12 |
| 别名 | HOPA, FGS1, CAGH45, CAG repeat protein 45, ARC240 |
| 计算分子量 | 243 kDa |
| 观测分子量 | 243 kDa |
| GenBank蛋白编号 | NM_005120 |
| 基因名称 | MED12 |
| Gene ID (NCBI) | 9968 |
| RRID | AB_10644141 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q93074 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED12 antibody 20028-1-AP | Download protocol |
| IF protocol for MED12 antibody 20028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. | ||
Mod Pathol MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma. | ||
Genes (Basel) MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.
| ||
Pathol Res Pract Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma. | ||
Naunyn Schmiedebergs Arch Pharmacol Loureirin B improves H/R-induced hepatic ischemia-reperfusion injury by downregulating ALOX5 to regulate mitochondrial homeostasis |
