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  • Featured Product
  • KD/KO Validated

MECP2 Polyclonal antibody

MECP2 Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC,IF-P, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10861-1-AP

Print datasheet

Synonyms

AUTSX3, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

验证数据展示

  • IP experiment of HEK-293T using 10861-1-AP

    IP experiment of HEK-293T using 10861-1-AP

    IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with HEK-293T cells lysate 1640 ug.

  • WB analysis of MDA-MB-453s using 10861-1-AP

    WB analysis of MDA-MB-453s using 10861-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 10861-1-AP

    WB analysis using 10861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10861-1-AP

    IP experiment of MCF-7 using 10861-1-AP

    IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.

  • IHC staining of human brain using 10861-1-AP

    IHC staining of human brain using 10861-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10861-1-AP (MECP2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10861-1-AP

    IF Staining of HepG2 using 10861-1-AP

    Immunofluorescent analysis of HepG2 cells using 10861-1-AP (MECP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of NIH/3T3 using 10861-1-AP

    FC experiment of NIH/3T3 using 10861-1-AP

    1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (10861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

  • IHC staining of rat brain using 10861-1-AP

    IHC staining of rat brain using 10861-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 10861-1-AP

    IHC staining of rat brain using 10861-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 10861-1-AP

    IF Staining of mouse cerebellum using 10861-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 10861-1-AP

    IF Staining of mouse cerebellum using 10861-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMDA-MB-453s cells, human brain tissue, MCF-7 cells, mouse heart tissue, mouse lung tissue, SH-SY5Y cells, mouse ovary tissue, mouse brain tissue, rat lung tissue
Positive IP detected inMCF-7 cells, HEK-293T cells
Positive IHC detected inhuman brain tissue, rat brain tissue, human gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse cerebellum tissue
Positive FC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10861-1-AP targets MECP2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Cited Applications ChIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MECP2 fusion protein Ag1309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyl CpG binding protein 2 (Rett syndrome)
Synonyms AUTSX3, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Calculated Molecular Weight 52 kDa, 53 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC011612
Gene Symbol MECP2
Gene ID (NCBI) 4204
RRIDAB_2143843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51608
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

实验方案

Product Specific Protocols
WB protocol for MECP2 antibody 10861-1-APDownload protocol
IHC protocol for MECP2 antibody 10861-1-APDownload protocol
IF protocol for MECP2 antibody 10861-1-APDownload protocol
IP protocol for MECP2 antibody 10861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseChIP

Science

MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.

Authors - Abdulkhaleg Ibrahim
View Article
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
View Article
humanWB, IF

Clin Immunol

Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2.

Authors - Anamika Basu
View Article
mouse,humanWB

Br J Pharmacol

Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.

Authors - Ai Wei
View Article
ratWB

Int J Biol Macromol

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.

Authors - Ruike Wang
View Article
humanWB

Mol Cancer Res

The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.

Authors - Leoh Lai Sum LS
View Article