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验证数据展示

  • WB analysis of Jurkat using 10267-1-AP

    WB analysis of Jurkat using 10267-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10267-1-AP

    IHC staining of human pancreas cancer using 10267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10267-1-AP (MEA1 antibody) at dilution of 1:50 (under 40x lens).

MEA1 Polyclonal antibody

MEA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10267-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HYS, male enhanced antigen 1, MEA, MEA 1, MEA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10267-1-AP targets MEA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0375

Product name: Recombinant human MEA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-185 aa of BC001754

Sequence: MGPERHLSGAPARMATVVLGGDTMGPERIFPNQTEELGHQGPSEGTGDWSSEEPEEEQEETGSGPAGYSYQPLNQDPEQEEVELAPVGDGDVVADIQDRIQALGLHLPDPPLESEDEDEEGATALNNHSSIPMDPEHVELVKRTMAGVSLPAPGVPAWAREISDAQWEDVVQKALQARQASPAWK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 male-enhanced antigen 1
别名 HYS, male enhanced antigen 1, MEA, MEA 1, MEA1
计算分子量 20 kDa
观测分子量 34 kDa and 36 kDa
GenBank蛋白编号BC001754
基因名称 MEA1
Gene ID (NCBI) 4201
RRIDAB_2143838
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16626
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEA1, also named Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development. Endogenous protein was detected as two bands (34 kDa and 36 kDa). (PMID: 12444059)

实验方案

Product Specific Protocols
WB protocol for MEA1 antibody 10267-1-APDownload protocol
IHC protocol for MEA1 antibody 10267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols