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验证数据展示

  • WB analysis using 16619-1-AP (same clone as 16619-1-PBS)

    WB analysis using 16619-1-AP (same clone as 16619-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

    IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

    IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

    IP experiment of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

    IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:2000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16619-1-AP (same clone as 16619-1-PBS)

    IF Staining of HeLa using 16619-1-AP (same clone as 16619-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ME1 antibody (16619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ME1 Polyclonal antibody, PBS Only

ME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16619-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16619-1-PBS targets ME1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9916

Product name: Recombinant human ME1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 226-572 aa of BC025246

Sequence: DEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 malic enzyme 1, NADP(+)-dependent, cytosolic
别名 EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME
计算分子量 572 aa, 64 kDa
观测分子量 55-64 kDa
GenBank蛋白编号BC025246
基因名称 ME1
Gene ID (NCBI) 4199
RRIDAB_2143821
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48163
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

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