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验证数据展示

  • WB analysis using 28747-1-AP

    WB analysis using 28747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 28747-1-AP

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of MCF-7 using 28747-1-AP

    IP experiment of MCF-7 using 28747-1-AP

    IP result of anti-MDMX (IP:28747-1-AP, 4ug; Detection:28747-1-AP 1:3000) with MCF-7 cells lysate 1470 ug.

  • IHC staining of human breast cancer using 28747-1-AP

    IHC staining of human breast cancer using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 28747-1-AP

    IHC staining of human ovary tumor using 28747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MDMX/MDM4 Polyclonal antibody

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 28747-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA2780 cells, MCF-7 cells, MDA-MB-231 cells, U2OS cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28747-1-AP targets MDMX/MDM4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 MDMX/MDM4 fusion protein Ag30456 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Mdm4 p53 binding protein homolog (mouse)
别名 MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX
计算分子量 490 aa, 55 kDa
观测分子量 70-76 kDa
GenBank蛋白编号BC067299
基因名称 MDMX/MDM4
Gene ID (NCBI) 4194
RRIDAB_3086083
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IHC protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
IP protocol for MDMX/MDM4 antibody 28747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Sci

SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA

Authors - Shiyi Yu
View Article
humanWB

J Med Chem

Design of Murine Double Minute 2 Proteolysis Targeting Chimera Degraders with a Built-In Tumor-Targeting Ability

Authors - Zhuqian Wang
View Article