验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Raji cells, HepG2 cells, Apoptosised HeLa cells, A375 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17914-1-AP targets MDMX/MDM4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MDMX/MDM4 fusion protein Ag12319 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Mdm4 p53 binding protein homolog (mouse) |
| 别名 | MDM4, MDMX, HDMX, Mdm2-like p53-binding protein, p53-binding protein Mdm4 |
| 计算分子量 | 490 aa, 55 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC067299 |
| 基因名称 | MDMX/MDM4 |
| Gene ID (NCBI) | 4194 |
| RRID | AB_2143683 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MDMX/MDM4 antibody 17914-1-AP | Download protocol |
| IHC protocol for MDMX/MDM4 antibody 17914-1-AP | Download protocol |
| IP protocol for MDMX/MDM4 antibody 17914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration. | ||
Cancer Res FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX. | ||
Redox Biol Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor. | ||
Cell Death Discov LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX | ||
J Med Chem Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells. | ||
PLoS Genet MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.
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