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验证数据展示

  • FC experiment of A431 using 83141-5-RR

    FC experiment of A431 using 83141-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Mdm1 Recombinant Antibody (83141-5-RR, Clone:230425E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83141-5-RR

    Affinity and Kinetic Characterization of 83141-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83141-5-RR against Human MDM1 were performed. The affinity constant is 0.563 nM.

MDM1 Recombinant monoclonal antibody

MDM1 Uni-rAb® Recombinant Antibody for FC (Intra), ELISA
Cat No. 83141-5-RR

产品说明书

CloneNo. 230425E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

Nuclear protein MDM1, 230425E2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83141-5-RR targets MDM1 in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10541

Product name: Recombinant human MDM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 365-714 aa of BC028355

Sequence: SDSNCCWDVSSTTSSEGTVSSNIRALDLAGDPTSHKTLQKCPSTEPEEKGNIVEEQPQKNTTEKLGVSAPTIPVRRRLAWDTENTSEDVQKQPGEKEEEDDNEEEGDRKTGKQAFMGEQEKLDVHEKSKADKMKEGSDSSVSSEKGGRLPTPKLRELGGIQRTHHDLTTPAVGGAVLVSPSKMKPPALEQRKRMTSQDCLETSKNDFTKKESRAVSLLTSPAAGIKTVDPLPLREDSEDNIHKFAEATLPVSKIPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKENFWGKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Mdm1 nuclear protein homolog (mouse)
别名 Nuclear protein MDM1, 230425E2
计算分子量 81 kDa
GenBank蛋白编号BC028355
基因名称 MDM1
Gene ID (NCBI) 56890
RRIDAB_3670842
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TC05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mdm1 is highly conserved in mammals as four sorting nexins (Snx): Snx13, 14, 19, and 25. Snx14 alleles are implicated in an autosomal-recessive neurological disease characterized by cellular vacuolization and granular plaque accumulation in late endosomes or autophagosomes. (PMID: 26283797)

实验方案

Product Specific Protocols
FC protocol for MDM1 antibody 83141-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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