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验证数据展示

  • WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

    WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84576-4-RR

    Affinity and Kinetic Characterization of 84576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84576-4-RR against Human MDH1 were performed. The affinity constant is below 1 pM.

MDH1 Recombinant monoclonal antibody, PBS Only

MDH1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84576-4-PBS

产品说明书

CloneNo. 241887A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84576-4-PBS targets MDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8744

Product name: Recombinant human MDH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-334 aa of BC001484

Sequence: MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 malate dehydrogenase 1, NAD (soluble)
别名 MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase
计算分子量 334 aa, 36 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC001484
基因名称 MDH1
Gene ID (NCBI) 4190
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP40925
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

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