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  • Featured Product
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MDH1 Monoclonal antibody

MDH1 Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9A2

Cat No : 66505-1-Ig

Print datasheet

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

验证数据展示

  • WB analysis using 66505-1-Ig

    WB analysis using 66505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66505-1-Ig

    WB analysis of HEK-293 using 66505-1-Ig

    WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

  • IF Staining of HepG2 using 66505-1-Ig

    IF Staining of HepG2 using 66505-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HEK-293 using 66505-1-Ig

    FC experiment of HEK-293 using 66505-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cells
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66505-1-Ig targets MDH1 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen MDH1 fusion protein Ag8744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name malate dehydrogenase 1, NAD (soluble)
Synonyms Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 36-37 kDa
GenBank Accession NumberBC001484
Gene Symbol MDH1
Gene ID (NCBI) 4190
RRIDAB_2881868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)

实验方案

Product Specific Protocols
WB protocol for MDH1 antibody 66505-1-IgDownload protocol
IF protocol for MDH1 antibody 66505-1-IgDownload protocol
FC protocol for MDH1 antibody 66505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols