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验证数据展示

  • WB analysis of mouse liver using 17578-1-AP

    WB analysis of mouse liver using 17578-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17578-1-AP (MCTP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17578-1-AP

    IHC staining of human brain using 17578-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17578-1-AP (MCTP2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17578-1-AP

    IHC staining of human brain using 17578-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17578-1-AP (MCTP2 antibody) at dilution of 1:100 (under 40x lens).

MCTP2 Polyclonal antibody

MCTP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17578-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MCTP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, human placenta tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17578-1-AP targets MCTP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11089

Product name: Recombinant human MCTP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 4-306 aa of BC025708

Sequence: LDIEVWGKDNKKHEERLGTCKVDISALPLKQANCLELPLDSCLGALLMLVTLTPCAGVSVSDLCVCPLADLSERKQITQRYCLQNSLKDVKDVGILQVKVLKAADLLAADFSGKSDPFCLLELGNDRLQTHTVYKNLNPEWNKVFTFPIKDIHDVLEVTVFDEDGDKPPDFLGKVAIPLLSIRDGQPNCYVLKNKDLEQAFKGVIYLEMDLIYNPVKASIRTFTPREKRFVEDSRKLSKKILSRDVDRVKRITMAIWNTMQFLKSCFQWESTLRSTIAFAHRKEEPPLSMKCTLFSGDIGPSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 multiple C2 domains, transmembrane 2
别名 MCTP2
计算分子量 878 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC025708
基因名称 MCTP2
Gene ID (NCBI) 55784
RRIDAB_2143118
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6DN12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCTP2 (Multiple C2 domains with two transmembrane regions 2) is involved in intercellular signal transduction and synapse function. MCTP2 may be a genetic cause of CoA and related cardiac malformations.

实验方案

Product Specific Protocols
WB protocol for MCTP2 antibody 17578-1-APDownload protocol
IHC protocol for MCTP2 antibody 17578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols