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验证数据展示

  • WB analysis using 84481-4-RR

    WB analysis using 84481-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84481-4-RR (MCSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84481-4-RR

    Affinity and Kinetic Characterization of 84481-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84481-4-RR against Human M-CSF were performed. The affinity constant is 11.4 nM.

M-CSF Recombinant antibody

M-CSF Recombinant Antibody for WB, ELISA
Cat No. 84481-4-RR

产品说明书

CloneNo. 241743C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

M CSF, Lanimostim, CSF-1, CSF1, CSF 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84481-4-RR targets M-CSF in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 1 (macrophage)
别名 M CSF, Lanimostim, CSF-1, CSF1, CSF 1
计算分子量 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC021117
基因名称 M-CSF
Gene ID (NCBI) 1435
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09603
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

实验方案

Product Specific Protocols
WB protocol for M-CSF antibody 84481-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols