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验证数据展示

  • WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

    WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

    IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51608-1

    Cytometric bead array sample test of MP51608-1

    Cytometric bead array sample test of MP51608-1, MCM5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67049-1-PBS. Detection antibody: 67049-2-PBS.

  • Cytometric bead array standard curve of MP51608-1

    Cytometric bead array standard curve of MP51608-1

    Cytometric bead array standard curve of MP51608-1, MCM5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67049-1-PBS. Detection antibody: 67049-2-PBS. Standard:Ag28704. Range: 0.781-100 ng/mL.

MCM5 Monoclonal antibody, PBS Only (Capture)

MCM5 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67049-1-PBS

产品说明书

CloneNo. 3B7F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

3B7F8, CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MCM5 Ready-To-Use IHC Kit. MCM5 primary antibody included.

产品信息

67049-1-PBS targets MCM5 as part of a matched antibody pair:

MP51608-1: 67049-1-PBS capture and 67049-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28704

Product name: Recombinant human MCM5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 388-734 aa of BC000142

Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 5
别名 3B7F8, CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46
计算分子量 82 kDa
观测分子量 82-88 kDa
GenBank蛋白编号BC000142
基因名称 MCM5
Gene ID (NCBI) 4174
RRIDAB_2882362
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP33992
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

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