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验证数据展示

  • WB analysis of human liver using 12117-1-AP (same clone as 12117-1-PBS)

    WB analysis of human liver using 12117-1-AP (same clone as 12117-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12117-1-AP (MCCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12117-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12117-1-AP (same clone as 12117-1-PBS)

    IHC staining of human pancreas cancer using 12117-1-AP (same clone as 12117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12117-1-AP (MCCC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12117-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 12117-1-AP (same clone as 12117-1-PBS)

    IP experiment of mouse kidney using 12117-1-AP (same clone as 12117-1-PBS)

    IP result of anti-MCCC2 (IP:12117-1-AP, 4ug; Detection:12117-1-AP 1:300) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 12117-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MCCC2 Polyclonal antibody, PBS Only

MCCC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12117-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12117-1-PBS targets MCCC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2767

Product name: Recombinant human MCCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC014897

Sequence: MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGIKPLEKHKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
别名
计算分子量 563 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC014897
基因名称 MCCC2
Gene ID (NCBI) 64087
RRIDAB_2143810
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCC0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCCC2(Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial) is also named as MCCB and belongs to the AccD/PCCB family. The putative 563-amino acid polypeptide has a calculated molecular mass of 61 kD and contains an N-terminal mitochondrial targeting sequence(PMID:11181649). It catalyzes carboxylation of 3 methylcrotonyl COA to form 3 methylglutaconyl-COA and is probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits. Defects in MCCC2 are the cause of methylcrotonoyl-CoA carboxylase 2 deficiency (MCC2D).

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