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验证数据展示

  • WB analysis using 30081-1-AP (same clone as 30081-1-PBS)

    WB analysis using 30081-1-AP (same clone as 30081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30081-1-AP (MCCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 30081-1-AP (same clone as 30081-1-PBS)

    IHC staining of human ovary cancer using 30081-1-AP (same clone as 30081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30081-1-AP (same clone as 30081-1-PBS)

    IHC staining of human stomach cancer using 30081-1-AP (same clone as 30081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30081-1-AP (same clone as 30081-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 30081-1-AP (same clone as 30081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 30081-1-AP (same clone as 30081-1-PBS)

    IF Staining of U-251 using 30081-1-AP (same clone as 30081-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 30081-1-AP (same clone as 30081-1-PBS)

    IF Staining of MCF-7 using 30081-1-AP (same clone as 30081-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

MCCC1 Polyclonal antibody, PBS Only

MCCC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30081-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30081-1-PBS targets MCCC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32459

Product name: Recombinant human MCCC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-200 aa of BC004187

Sequence: TTATGRNITKVLIANRGEIACRVMRTAKKLGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAELCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
别名 MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit
计算分子量 76 kDa
观测分子量70 kDa
GenBank蛋白编号BC004187
基因名称 MCCC1
Gene ID (NCBI) 56922
RRIDAB_3086226
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RQ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

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