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验证数据展示

  • WB analysis using 15536-1-AP

    WB analysis using 15536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15536-1-AP (MCAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15536-1-AP

    IF Staining of HepG2 using 15536-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCAT antibody (15536-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MCAT Polyclonal antibody

MCAT Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15536-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

MCT, Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, FASN2C, fabD, EC:2.3.1.39

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15536-1-AP targets MCAT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7867

Product name: Recombinant human MCAT protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-390 aa of BC042195

Sequence: MSVRVARVAWVRGLGASYRRGASSFPVPPPGAQGVAELLRDATGAEEEAPWAATERRMPGQCSVLLFPGQGSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQPAIFVASLAAVEKLHHLQPSVIENCVAAAGFSVGEFAALVFAGAMEFAEGLYAVKIRAEAMQEASEAVPSGMLSVLGQPQSKFNFACLEAREHCKSLGIENPVCEVSNYLFPDCRVISGHQEALRFLQKNSSKFHFRRTRMLPVSGAFHTRLMEPAVEPLTQALKAVDIKKPLVSVYSNVHGHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGPGRQLGAILKSCNMQAWKSYSAVDVLQTLEHVDLDPQEPPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 malonyl CoA:ACP acyltransferase (mitochondrial)
别名 MCT, Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, FASN2C, fabD, EC:2.3.1.39
计算分子量 43 kDa
观测分子量39 kDa
GenBank蛋白编号BC042195
基因名称 MCAT
Gene ID (NCBI) 27349
RRIDAB_3669211
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Malonyl-CoA-acyl carrier protein transacylase (MCAT), also known as MT, is an enzyme involved in mitochondrial fatty acid synthesis (mtFAS) and catalyzes the transfer of the malonyl moiety of malonyl-CoA to the mitochondrial acyl carrier protein (ACP) (PMID: 36881526). It has four coding exons and is transcribed into two isoforms by the alternative splicing of exon 3 (PMID: 33918393).

实验方案

Product Specific Protocols
IF protocol for MCAT antibody 15536-1-APDownload protocol
WB protocol for MCAT antibody 15536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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