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验证数据展示

  • IHC staining of mouse testis using 83546-3-RR

    IHC staining of mouse testis using 83546-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MBOAT7 antibody included
  • IHC staining of mouse testis using 83546-3-RR

    IHC staining of mouse testis using 83546-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MBOAT7 antibody included
  • IF Staining of HuH-7 using 83546-3-RR

    IF Staining of HuH-7 using 83546-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83546-3-RR

    FC experiment of A549 using 83546-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83546-3-RR

    Affinity and Kinetic Characterization of 83546-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

MBOAT7 Recombinant monoclonal antibody

MBOAT7 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83546-3-RR

产品说明书

CloneNo. 240505E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

1-acylglycerophosphatidylinositol O-acyltransferase, 240505E8, BB1, Bladder and breast carcinoma-overexpressed gene 1 protein, EC:2.3.1.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHuH-7 cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MBOAT7 Ready-To-Use IHC Kit. MBOAT7 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83546-3-RR targets MBOAT7 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24466

Product name: Recombinant human MBOAT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-344 aa of BC006309

Sequence: SSPEKAASLEYDYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane bound O-acyltransferase domain containing 7
别名 1-acylglycerophosphatidylinositol O-acyltransferase, 240505E8, BB1, Bladder and breast carcinoma-overexpressed gene 1 protein, EC:2.3.1.-
计算分子量 472 aa, 53 kDa
GenBank蛋白编号BC006309
基因名称 MBOAT7
Gene ID (NCBI) 79143
RRIDAB_3671165
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96N66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

实验方案

Product Specific Protocols
FC protocol for MBOAT7 antibody 83546-3-RRDownload protocol
IF protocol for MBOAT7 antibody 83546-3-RRDownload protocol
IHC protocol for MBOAT7 antibody 83546-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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