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验证数据展示

  • WB analysis using 66837-1-Ig (same clone as 66837-1-PBS)

    WB analysis using 66837-1-Ig (same clone as 66837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBNL1 antibody included
  • IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBNL1 antibody included
  • IF Staining of MCF-7 using 66837-1-Ig (same clone as 66837-1-PBS)

    IF Staining of MCF-7 using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBNL1 antibody (66837-1-Ig, Clone: 1H2C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

MBNL1 Monoclonal antibody, PBS Only

MBNL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66837-1-PBS

产品说明书

CloneNo. 1H2C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

1H2C12, EXP, EXP35, EXP40, EXP42

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MBNL1 Ready-To-Use IHC Kit. MBNL1 primary antibody included.

产品信息

66837-1-PBS targets MBNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag7752

Product name: Recombinant human MBNL1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-343 aa of BC050535

Sequence: MGRCSRENCKYLHPPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFSVAPSLATNASAAAFNPYLGPVSPSLVPAEILPTAPMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAHPADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMTQSAVKSLKRPLEATFDLGIPQAVLPPLPKRPALEKTNGATAVFNTGIFQYQQALANMQLQQHTAFLPPGSILCMTPATSVVPMVHGATPATVSAATTSATSVPFAATATANQIPIISAEHLTSHKYVTQM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 muscleblind-like (Drosophila)
别名 1H2C12, EXP, EXP35, EXP40, EXP42
计算分子量 42 kDa
观测分子量 40 kDa
GenBank蛋白编号BC050535
基因名称 MBNL1
Gene ID (NCBI) 4154
RRIDAB_2882180
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR56
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.

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