验证数据展示
经过测试的应用
| Positive WB detected in | human skin tissue, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
11270-1-AP targets MBD4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1822 Product name: Recombinant human MBD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-574 aa of BC011752 Sequence: VRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methyl-CpG binding domain protein 4 |
| 别名 | EC:3.2.2.-, MED1, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC011752 |
| 基因名称 | MBD4 |
| Gene ID (NCBI) | 8930 |
| RRID | AB_2139871 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95243 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MBD4 antibody 11270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
| ||
Genet Test Mol Biomarkers The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas. |
