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验证数据展示

  • WB analysis using 86681-2-RR

    WB analysis using 86681-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86681-2-RR (MBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86681-2-RR

    IF Staining of HeLa using 86681-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD3 antibody (86681-2-RR, Clone: 250922E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MBD3 Recombinant monoclonal antibody

MBD3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86681-2-RR

产品说明书

CloneNo. 250922E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MBD 3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, Y79 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86681-2-RR targets MBD3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5531

Product name: Recombinant human MBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-259 aa of BC043619

Sequence: MERKSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyl-CpG binding domain protein 3
别名 MBD 3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3
计算分子量 33 kDa
观测分子量35 kDa
GenBank蛋白编号BC043619
基因名称 MBD3
Gene ID (NCBI) 53615
RRIDAB_3745051
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95983
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methyl-CpG-binding domain 3 (Mbd3), a member among the five-MBD protein family, is unique in its ability to specifically recognize 5′-hydroxymethyl-cytosine (5′-hmC), an epigenetic marker that is highly enriched in stem cells and cancer.

实验方案

Product Specific Protocols
IF protocol for MBD3 antibody 86681-2-RRDownload protocol
WB protocol for MBD3 antibody 86681-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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