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验证数据展示

  • WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    WB result of MAVS; VISA antibody (81910-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAVS antibody included
  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAVS antibody included
  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81910-1-RR

    Affinity and Kinetic Characterization of 81910-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is 0.338 nM.

  • Featured Product
  • KD/KO验证

MAVS; VISA Recombinant monoclonal antibody, PBS Only

MAVS; VISA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 81910-1-PBS

产品说明书

CloneNo. 7F14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MAVS, 7F14, Cardif, IPS 1, IPS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAVS Ready-To-Use IHC Kit. MAVS primary antibody included.

产品信息

81910-1-PBS targets MAVS; VISA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5655

Product name: Recombinant human MAVS; VISA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC044952

Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial antiviral signaling protein
别名 MAVS, 7F14, Cardif, IPS 1, IPS1
计算分子量 57 kDa
观测分子量75 kDa
GenBank蛋白编号BC044952
基因名称 MAVS
Gene ID (NCBI) 57506
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z434
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

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