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验证数据展示

  • WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    WB analysis of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    WB result of MAVS; VISA antibody (81910-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAVS antibody included
  • IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAVS antibody included
  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81910-1-RR

    Affinity and Kinetic Characterization of 81910-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is 0.338 nM.

  • Featured Product
  • KD/KO Validated

MAVS; VISA Recombinant monoclonal antibody, PBS Only

MAVS; VISA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 81910-1-PBS

产品说明书

CloneNo. 7F14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MAVS, 7F14, Cardif, IPS 1, IPS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAVS Ready-To-Use IHC Kit. MAVS primary antibody included.

产品信息

81910-1-PBS targets MAVS; VISA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5655

Product name: Recombinant human MAVS; VISA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC044952

Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial antiviral signaling protein
别名 MAVS, 7F14, Cardif, IPS 1, IPS1
计算分子量 57 kDa
观测分子量75 kDa
GenBank蛋白编号BC044952
基因名称 MAVS
Gene ID (NCBI) 57506
RRIDAB_2935592
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z434
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

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