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验证数据展示

  • IHC staining of human lung cancer using 12202-2-AP

    IHC staining of human lung cancer using 12202-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MATR3 antibody included
  • IF Staining of SH-SY5Y using 12202-2-AP

    IF Staining of SH-SY5Y using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HEK-293 using 12202-2-AP

    IF Staining of HEK-293 using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 12202-2-AP

    IHC staining of mouse testis using 12202-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MATR3 antibody included
  • WB analysis of MCF-7 using 12202-2-AP

    WB analysis of MCF-7 using 12202-2-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12202-2-AP (MATR3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 12202-2-AP

    IF Staining of HEK-293 using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (12202-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MATR3 Polyclonal antibody

MATR3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12202-2-AP

产品说明书

引用文献(15)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

Matrin-3, matrin 3, KIAA0723

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MATR3 Ready-To-Use IHC Kit. MATR3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12202-2-AP targets MATR3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag2843

Product name: Recombinant human MATR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 495-847 aa of BC015031

Sequence: GRVIHLSNLPHSGYSDSAVLKLAEPYGKIKNYILMRMKSQAFIEMETREDAMAMVDHCLKKALWFQGRCVKVDLSEKYKKLVLRIPNRGIDLLKKDKSRKRSYSPDGKESPSDKKSKTDGSQKTESSTEGKEQEEKSGEDGEKDTKDDQTEQEPNMLLESEDELLVDEEEAAALLESGSSVGDETDLANLGDVASDGKKEPSDKAVKKDGSASAAAKKKLKKVDKIEELDQENEAALENGIKNEENTEPGAESSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrin 3
别名 Matrin-3, matrin 3, KIAA0723
计算分子量 847 aa, 95 kDa
观测分子量125 kDa
GenBank蛋白编号BC015031
基因名称 MATR3
Gene ID (NCBI) 9782
RRIDAB_2281752
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43243
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

实验方案

Product Specific Protocols
WB protocol for MATR3 antibody 12202-2-APDownload protocol
IHC protocol for MATR3 antibody 12202-2-APDownload protocol
IF protocol for MATR3 antibody 12202-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanWB

Nat Commun

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

Authors - Song Zhu
View Article
humanWB

Brain

MATR3 haploinsufficiency and early-onset neurodegeneration.

Authors - Michael Zech
View Article
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article
humanWB

Cell Rep

SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites

Authors - Bao Wang
View Article
humanWB

RNA Biol

Modulation of PDCD1 exon 3 splicing.

Authors - Junjie Sun
View Article