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验证数据展示

  • WB analysis using 67643-1-Ig

    WB analysis using 67643-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67643-1-Ig (MAT2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human colon cancer using 67643-1-Ig

    IHC staining of human colon cancer using 67643-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67643-1-Ig (MAT2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAT2B antibody included

MAT2B Monoclonal antibody

MAT2B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67643-1-Ig

产品说明书

CloneNo. 2B7G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

2B7G8, MAT II, MAT II beta, MATIIbeta, MSTP045

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, NIH/3T3 cells, HSC-T6 cells, LNcaP cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAT2B Ready-To-Use IHC Kit. MAT2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67643-1-Ig targets MAT2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8649

Product name: Recombinant human MAT2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-323 aa of BC005218

Sequence: MPEMPEDMEQEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNTQLDCSKLETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 methionine adenosyltransferase II, beta
别名 2B7G8, MAT II, MAT II beta, MATIIbeta, MSTP045
计算分子量 36 kDa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC005218
基因名称 MAT2B
Gene ID (NCBI) 27430
RRIDAB_2882843
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZL9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MAT2B antibody 67643-1-IgDownload protocol
WB protocol for MAT2B antibody 67643-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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