验证数据展示

WB analysis using 55309-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55309-1-AP (MAT2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55309-1-AP
WB result of MAT2A antibody (55309-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-control and sh-MAT2A transfected HeLa cells.
IF Staining of HeLa using 55309-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT2A antibody (55309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human stomach cancer using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included
IP experiment of HepG2 using 55309-1-AP
IP result of anti-MAT2A (IP:55309-1-AP, 4ug; Detection:55309-1-AP 1:500) with HepG2 cells lysate 3200ug.
IHC staining of human kidney using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included
IHC staining of human colon cancer using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included
IHC staining of human stomach cancer using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included
IHC staining of mouse liver using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included
IHC staining of rat kidney using 55309-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MAT2A antibody included

Featured Product
KD/KO验证

MAT2A Polyclonal antibody

MAT2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55309-1-AP

产品说明书

引用文献(21)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (21)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy MAT2A Ready-To-Use IHC Kit. MAT2A primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 4 publications below

产品信息

55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	methionine adenosyltransferase II, alpha
别名	AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII
计算分子量	44 kDa
观测分子量	47 kDa, 49 kDa
GenBank蛋白编号	NM_005911
基因名称	MAT2A
Gene ID (NCBI)	4144
RRID	AB_2881303
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P31153
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

实验方案

Product Specific Protocols
IF protocol for MAT2A antibody 55309-1-AP	Download protocol
IHC protocol for MAT2A antibody 55309-1-AP	Download protocol
IP protocol for MAT2A antibody 55309-1-AP	Download protocol
WB protocol for MAT2A antibody 55309-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa KD Validated View Article
human	WB,IF	Oxid Med Cell Longev Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16. Authors - Peng-Bo Chen KD Validated View Article
pig	WB	Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide Authors - Menghui Xu View Article
mouse	WB	FASEB J Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration. Authors - Li Chen KD Validated View Article
mouse	WB,IF	FASEB J Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss. Authors - Honglei Kang KD Validated View Article
rat	WB	Environ Toxicol Pharmacol Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium. Authors - Wenhua Kuang View Article

验证数据展示

WB analysis using 55309-1-AP

WB analysis of HeLa using 55309-1-AP

IF Staining of HeLa using 55309-1-AP

IHC staining of human stomach cancer using 55309-1-AP

IP experiment of HepG2 using 55309-1-AP

IHC staining of human kidney using 55309-1-AP

IHC staining of human colon cancer using 55309-1-AP

IHC staining of human stomach cancer using 55309-1-AP

IHC staining of mouse liver using 55309-1-AP

IHC staining of rat kidney using 55309-1-AP

MAT2A Polyclonal antibody

MAT2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.

Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.

Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.